Club Yeast Bank?

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Club Yeast Bank?

Postby HarvInSTL » Wed May 30, 2007 2:27 pm

After paying about $7 for the grains in my last 10gal batch and about $15 for my yeast I am looking long and hard at yeast slanting.

I've got a seperate dorm fridge that i will be using for just yeast, and the wife is a chemistry teacher part time so she has the connections on cheap screw cap culture tubes.

Other than that its just the time and effort for me to get started. At first I am going to start by making 5-10 slants for each yeast type that I purchase.

Would anyone be interested in joining in on this adventure?
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Postby blksabbath » Wed May 30, 2007 9:54 pm

I've got about 10 or so strains on slants. Although the past year or so I've been slacking and using dry yeast for most things. Anyways, I need to restreak everything I've got and can easily make extras if you want.
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Postby HarvInSTL » Wed May 30, 2007 10:05 pm

blksabbath wrote:I've got about 10 or so strains on slants. Although the past year or so I've been slacking and using dry yeast for most things. Anyways, I need to restreak everything I've got and can easily make extras if you want.
dan


Hells yeah!

What size tubes do you use for your slants?

I found 100 16mmx125mm glass culture tubs with screw caps that can be autoclaved/pressure cooked for $39. I will prolly order them next week.

http://www.thegrape.net/browse.cfm/4,9723.htm

They also sell some with agar in them, but they are $1.95 each if you do that. While I only need at most 2 or 3 cultures of each strain, I figured that hopefully I can build up a decent size yeast bank that any member could have access to.

First up will be a special strain from white labs, wlp410!

http://www.whitelabs.com/beer/homebrew_strains_wlp410.html
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Postby JE » Thu May 31, 2007 7:18 am

Harv...let me know when you get that one ready. I might want to take a bit of that off your hands (have a Wit coming up soon)!

And Dan, why haven't you shared this information with me before...I keep buying all this darn Wyeast when I could just walk to your house!!!!
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Postby Benjamin R » Thu May 31, 2007 7:35 am

I would be happy to contribute $ or time to help this project (and save me 8 bucks a batch). Let me know what you need.
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Postby Konertjm » Thu May 31, 2007 8:02 am

Benjamin R wrote:I would be happy to contribute $ or time to help this project (and save me 8 bucks a batch). Let me know what you need.


As would I. I have always wanted to do some yeast ranching, but lacked knowledge, equipment, and space. Maybe some type of co-operative where each member had to contribute time, money, or whatever it took to support a yeast bank.

Edited for bad syntax :oops:
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Postby JE » Thu May 31, 2007 8:13 am

Hey, that's not a bad idea...the "Brew Bank" could be a great way to save us all some money! All we would need is a place to put all the equipment and the like, since we apparently have people 'in the know' on how to do it.

Then, just put in some time helping with things (perhaps some money to get the needed equipment) and we have yeast available when we need it.

Good plan...I'd be willing to assist in this too (although I also know absolutely nothing about it)!
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Postby blksabbath » Thu May 31, 2007 8:28 am

I found 100 16mmx125mm glass culture tubs with screw caps that can be autoclaved/pressure cooked for $39. I will prolly order them next week.


I think that's the size I have. About 15mls anyways. I got the agar from B3, but you can get it from lab supply places or even asian markets in a block. The only pain about working up from slants or small cultures is the actual stepping up process. I go from slant to 10mls to 50 mls to 250 then on and on from there. Takes a week or so to get enough for a healthy 5 gal pitch.

Look in the Brewnote archives for Tim Farhner's yeast culturing article. Tim also has lots of neat stuff frozen down.
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Postby HarvInSTL » Thu May 31, 2007 8:36 am

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Postby blksabbath » Thu May 31, 2007 9:22 am

Here's another really good reference:
http://www.maltosefalcons.com/tech/MB_R ... turing.php
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Postby OldTree » Thu May 31, 2007 9:27 am

I'll throw in my $0.02 here too. Below is a company that I usually see mentioned in homebrewing circles when it comes to scientific supplies.

http://www.cynmar.com

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Postby HarvInSTL » Mon Jun 04, 2007 6:26 pm

After talking to some of you guys yesterday, it seems that quite a few of you are receptive to the idea of maintaining a group yeast bank (or at least us having the ability to swap yeast slants).

I'm going to order the 100 screw top culture tubes, agar stuff this week.

Here is the yeast I currently have available or will have available soon.

Wyeast 1010 American Wheat Ale
Wyeast 1028 London Ale
Wyeast 3944 Belgian Witbier
White Labs WLP410 Belgian Witt II Ale
White labs WLP400
Wyeast 2633 Octoberfest Lager Blend
Wyeast 2278 Czech Pilsner Lager

I'll post an update once the supplies arrive.

If you have any slants that you are willing to contribute just let me know.

Thanks!
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Postby HarvInSTL » Tue Jun 19, 2007 3:37 pm

Just wanted to give everyone an update.

I've got the following items on order and just need to figure out which tubes to buy.

I bought the following for $38.26
2 Flasks, Erlynmeyer, Lighty Duty, 1000ml @ $2.47 = $4.94
1 Flasks, Erlynmeyer, Lighty Duty, 2000ml @ $4.97 = $4.97
2 Flasks, Erlynmeyer, Lighty Duty, 100ml @ $0.62 = $1.24
2 Flasks, Erlynmeyer, Lighty Duty, 250ml @ $0.87 = $1.74
1 Flasks, Erlynmeyer, Lighty Duty, 5000ml @ $12.47 = $12.47
2 Flasks, Erlynmeyer, Lighty Duty, 500ml @ $1.37 = $2.74
1 Thermometer Mercury -20-110C Yellow Back tot imm @ $3.00 = $3.00
1 TEST TUBE RACK PLASTIC For 40 tubes @ $2.81 = $2.81
1 ALCOHOL LAMP / BURNER Flint glass, 120 ml @ $2.78 = $2.78
1 Wicks For Alcohol Lamp Burner, 12/pk @ $1.57 = $1.57

Now I need to figure out which stir bars to buy and which culture tubes.

I'm torn between these for $30
http://www.onlinesciencemall.com/Shop/Control/Product/fp/SFV/30852/vpid/2037235/vpcsid/0/rid/119435

or these for $39.
http://www.thegrape.net/browse.cfm/4,9723.htm

Both can be autoclaved and those prices are without shipping. The agar should be here late this week, early next week and I already have about 4lbs of DME.

Thoughts?
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Postby StLBeer » Tue Jun 19, 2007 10:01 pm

I'd go for the glass ones. From what I know about microbiology, you want to flame the opening of the culture tube before inoculating. Can't do that with the polypropylene ones. There will be some breakage, but they can be returned.
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Postby StLBeer » Tue Jun 19, 2007 10:03 pm

Forgot to mention. I have 2 quarts of Wyeast 1056 American Ale slurry.
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